A simple, efficient, and reliable endoderm differentiation protocol for human embryonic stem cells using crotonate

Yi Fang; Xiaoling Li

doi:10.1016/j.xpro.2021.100659

A simple, efficient, and reliable endoderm differentiation protocol for human embryonic stem cells using crotonate

STAR Protoc. 2021 Jul 7;2(3):100659. doi: 10.1016/j.xpro.2021.100659. eCollection 2021 Sep 17.

Authors

Yi Fang¹, Xiaoling Li¹

Affiliation

¹ Signal Transduction Laboratory, National Institute of Environmental Health Sciences, Research Triangle Park, Durham, NC 27709, USA.

Abstract

Training and experiences are usually required to successfully culture and differentiate human embryonic stem cells (hESCs). Here, we describe a simple but highly efficient protocol to induce endoderm differentiation of hESCs with crotonate, a precursor of crotonyl-CoA for histone crotonylation deposition on endodermal genes. In this protocol, adding crotonate in different endoderm differentiation media significantly increases the differentiation efficiency and substantially reduces the amount of required reagents. For complete details on the use and execution of this protocol, please refer to Fang et al. (2021).

Keywords: Cell Biology; Cell Differentiation; Cell culture; Stem Cells; Tissue Engineering.

Publication types

Research Support, N.I.H., Intramural

MeSH terms

Cell Culture Techniques / methods*
Cell Differentiation / drug effects*
Cells, Cultured
Crotonates / pharmacology*
Endoderm / cytology*
Human Embryonic Stem Cells / drug effects*
Humans

Substances

Crotonates