Isolation of Cardiomyocytes Derived from Human Pluripotent Stem Cells Using miRNA Switches

Kenji Miki; Hirohide Saito; Yoshinori Yoshida

doi:10.1007/978-1-0716-1484-6_5

Isolation of Cardiomyocytes Derived from Human Pluripotent Stem Cells Using miRNA Switches

Methods Mol Biol. 2021:2320:35-51. doi: 10.1007/978-1-0716-1484-6_5.

Authors

Kenji Miki¹, Hirohide Saito², Yoshinori Yoshida³

Affiliations

¹ Department of Cell Growth and Differentiation, Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan.
² Department of Life Science Frontiers, Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan. [email protected].
³ Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan. [email protected].

PMID: 34302646
DOI: 10.1007/978-1-0716-1484-6_5

Abstract

The most common method for isolating cells of interest is an antibody method that recognizes cell surface antigens. However, specific surface antigens for many cell types have not been identified. We have developed the microRNA (miRNA)-responsive synthetic mRNA systems (miRNA switches), which isolate target cells based on endogenous miRNA activity. In this chapter, we describe protocols for isolating human pluripotent stem cell (hPSC)-derived cardiomyocytes using miRNA switches with or without cell sorting.

Keywords: Cardiomyocytes; Human pluripotent stem cells; Isolation; Synthetic modified mRNA; miRNA switch.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Culture Techniques / methods
Cell Differentiation / physiology
Cell Line
Cell Separation / methods
Flow Cytometry / methods
Humans
MicroRNAs / genetics*
Myocytes, Cardiac / cytology*
Pluripotent Stem Cells / cytology*
RNA, Messenger / genetics

Substances

MicroRNAs
RNA, Messenger