Voltammetric determination of uric acid using multiwall carbon nanotubes coated-poly(4-amino-3-hydroxy naphthalene sulfonic acid) modified glassy carbon electrode

Molla Tefera; Merid Tessema; Shimelis Admassie; Walelign Wubet

doi:10.1016/j.heliyon.2021.e07575

Voltammetric determination of uric acid using multiwall carbon nanotubes coated-poly(4-amino-3-hydroxy naphthalene sulfonic acid) modified glassy carbon electrode

Heliyon. 2021 Jul 15;7(7):e07575. doi: 10.1016/j.heliyon.2021.e07575. eCollection 2021 Jul.

Authors

Molla Tefera¹, Merid Tessema², Shimelis Admassie², Walelign Wubet¹

Affiliations

¹ Department of Chemistry, University of Gondar, P. O. Box 196, Gondar, Ethiopia.
² Department of Chemistry, Addis Ababa University, P. O. Box 1176, Addis Ababa, Ethiopia.

Abstract

In this study, an electrochemical sensor based multiwalled carbon nanotubes (MWCNTs)-poly (4-amino-3-hydroxy naphthalene sulfonic acid) modified glassy carbon electrode (MWCNTs/poly (AHNSA)/GCE) was developed for the determination of uric acid (UA). The composite electrode was prepared first by electropolymerization of the monomer (AHNSA) on GCE using cyclic voltammetry within the potential range of -0.8 V to +2.0 V vs Ag/AgCl for 15 cycles followed by drop coating of MWCNTs solution on the surface of poly (AHNSA)/GCE. Under optimal conditions, MWCNTs/poly (AHNSA)/GCE showed a linear current response with UA concentrations in the range of 1 × 10^-6 to 1 × 10^-4 M with R² = 0.9972. The sensor exhibited low detection limit with a value of 0.024 μM. The sensors have been applied to successfully quantify UA in urine samples.

Keywords: 4-amino-3-hydroxy naphthalene sulfonic acid; Multiwalled carbon nanotubes; Uric acid; Urine.