Comprehensive Analysis of the Expression, Prognosis, and Biological Significance of OVOLs in Breast Cancer

Jingsheng Chen; Hongjun Tang; Taidong Li; Kangwei Jiang; Haiming Zhong; Yuye Wu; Jiangtao He; Dongbing Li; Mengzhen Li; Xingsheng Cai

doi:10.2147/IJGM.S326402

Comprehensive Analysis of the Expression, Prognosis, and Biological Significance of OVOLs in Breast Cancer

Int J Gen Med. 2021 Jul 26:14:3951-3960. doi: 10.2147/IJGM.S326402. eCollection 2021.

Authors

Jingsheng Chen^#^{1

2}, Hongjun Tang^#¹, Taidong Li³, Kangwei Jiang², Haiming Zhong¹, Yuye Wu¹, Jiangtao He¹, Dongbing Li⁴, Mengzhen Li⁴, Xingsheng Cai⁴

Affiliations

¹ Department of Oncology, Central Hospital of Guangdong Nongken, Zhanjiang Cancer Hospital, Zhanjiang, 524002, Guangdong, People's Republic of China.
² Medical Department, Central Hospital of Guangdong Nongken, Zhanjiang Cancer Hospital, Zhanjiang, 524002, Guangdong, People's Republic of China.
³ Department of Thoracic Surgery, Central Hospital of Guangdong Nongken, Zhanjiang Cancer Hospital, Zhanjiang, 524002, Guangdong, People's Republic of China.
⁴ MyGene Diagnostics Co., Ltd, Guangzhou, 510000, Guangdong, People's Republic of China.

^# Contributed equally.

Abstract

Background: The study aimed to investigate the expression of OVOLs in breast cancer (BRCA) tissues and their value in prognosis.

Methods: ONCOMINE was used to analyze the expressions of OVOL1, OVOL2, and OVOL3 mRNA between BRCA tissues and normal breast tissues. The Wilcoxon rank sum test and t-test were used to assess the expression of OVOLs between BRCA tissues and unpaired/paired normal breast tissues. GEPIA and ROC curves were used to analyze the relationship between OVOLs expression and clinical pathological stage. Kaplan-Meier plotter was used to analyze prognosis. cBioPortal was used to analyze the mutation of OVOLs. GEPIA was used to analyze the co-expression of OVOLs. GO and KEGG analyses were performed by the DAVID software to predict the function of OVOLs co-expression genes.

Results: The expression of OVOL1/2 was significantly higher in BRCA tissues than in normal breast tissues. The OVOL3 expression correlated with tumor stage. The AUC of OVOLs was 0.757, 0.754, and 0.537, respectively. OVOL1 high expression was associated with shorter overall survival (HR: 1.48; 95% CI: 1.07-2.04; P=0.018). The OVOLs were associated with pathways including axon guidance, thyroid hormone signaling pathway, and ubiquinone and other terpenoid-quinone biosynthesis.

Conclusion: OVOL1 is a new potential marker of prognosis in BRCA, and OVOL1/2 are potential therapeutic targets in BRCA.

Keywords: OVOLs; bioinformatics; breast cancer; gene expression; prognostic.

Grants and funding

This work was supported by International Science and Technology Cooperation Project of Guangzhou Development Zone (2017GH12), and Pearl River S&T Nova Program of Guangzhou (201806010020 and 201906010020).