Interaction of the antitumor Au(I) complex [Au(Ph2P(CH2)2PPh2)2]Cl with human blood plasma, red cells, and lipoproteins: 31P and 1H NMR studies

J Inorg Biochem. 1987 Dec;31(4):267-81. doi: 10.1016/0162-0134(87)80081-8.

Abstract

The bis-chelated tetrahedral gold(I) complex [Au(dppe)2]Cl, where dppe is Ph2P(CH2)2PPh2, is active in several animal tumor models. When added to human blood plasma in vitro it appears to bind to lipoproteins, giving a slightly broadened 31P NMR signal, and 1H NMR resonances which are too broad to detect. Some lipoprotein is denatured. 31P NMR studies suggest that some [Au(dppe)2]+ is transferred from plasma to red cells with a half-life of ca. 2 hr. The complex binds within red cell membranes and the 1H resonances of intracellular glutathione are unaffected. The 31P NMR resonance from [Au(dppe)2]+ in red cell membranes is observable only when the complex is mobilized by addition of sodium dodecyl sulphate, which also mobilizes membrane phospholipids.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents / blood*
  • Chemical Precipitation
  • Erythrocytes / metabolism*
  • Humans
  • Hydrogen
  • Kinetics
  • Lipoproteins / blood*
  • Magnetic Resonance Spectroscopy / methods
  • Organogold Compounds
  • Organometallic Compounds / blood*
  • Organophosphorus Compounds / blood*
  • Phosphorus
  • Protein Binding

Substances

  • Antineoplastic Agents
  • Lipoproteins
  • Organogold Compounds
  • Organometallic Compounds
  • Organophosphorus Compounds
  • Phosphorus
  • bis(1,2-bis(diphenylphosphino)ethane)gold(I)
  • Hydrogen