Background: This study aims to describe immunophenotypic explorations at diagnosis and follow up of a pediatric patient with leukemic phase of ALK+ anaplastic large cell lymphoma (ALCL) by multiparametric flow cytometry (MFC).
Case: An 8-color MFC combination of antibodies allowed to identify neoplastic cells in concentrations until 0.02% during minimal residual disease (MRD) monitoring. Immunophenotypic shifts occurred in key markers as CD30, CD7, CD2, and CD5, however neoplastic cells were clearly discriminated from normal populations.
Conclusion: MFC can be a useful tool for ALCL diagnosis and MRD monitoring and may support therapeutic decisions.
Keywords: anaplastic large cell lymphoma; minimal residual disease; multiparameter flow cytometry.
© 2021 The Authors. Cancer Reports published by Wiley Periodicals LLC.