Generation of inducible pluripotent stem cell lines from Alzheimer's disease patients with APOE e3/e3 genotype

Jihye Lee; Zhong Liu; Ying Gai Tusing; Chao Li; Erik Westin; Wei Li; Rui Zhao

doi:10.1016/j.scr.2021.102498

Generation of inducible pluripotent stem cell lines from Alzheimer's disease patients with APOE e3/e3 genotype

Stem Cell Res. 2021 Aug:55:102498. doi: 10.1016/j.scr.2021.102498. Epub 2021 Aug 10.

Authors

Jihye Lee¹, Zhong Liu¹, Ying Gai Tusing¹, Chao Li¹, Erik Westin¹, Wei Li², Rui Zhao³

Affiliations

¹ Department of Biochemistry and Molecular Genetics, School of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
² Department of Clinical and Diagnoistic Sciences, School of Health Professions, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
³ Department of Biochemistry and Molecular Genetics, School of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294, USA. Electronic address: [email protected].

Abstract

iPSCs were generated from B lymphocytes of two Alzheimer's disease (AD) patients homozygous for the APOE e3 allele. The iPSCs express pluripotency-specific markers and have the capacity to differentiate into all three embryonic germ layers. Karyotyping analyses confirmed the iPSCs have normal karyotypes. These iPSCs can be utilized as an in vitro model to study AD and to evaluate efficacies of new treatments.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Alzheimer Disease* / genetics
Apolipoproteins E
Cell Differentiation
Genotype
Homozygote
Humans
Induced Pluripotent Stem Cells*

Substances

Apolipoproteins E

Abstract

Publication types

MeSH terms

Substances

Grants and funding