Comparative genomics of Enterobacter cloacae complex before and after acquired clinical resistance to Ceftazidime-Avibactam

Fiona Senchyna; Fiona B Tamburini; Kanagavel Murugesan; Nancy Watz; Ami S Bhatt; Niaz Banaei

doi:10.1016/j.diagmicrobio.2021.115511

Comparative genomics of Enterobacter cloacae complex before and after acquired clinical resistance to Ceftazidime-Avibactam

Diagn Microbiol Infect Dis. 2021 Dec;101(4):115511. doi: 10.1016/j.diagmicrobio.2021.115511. Epub 2021 Jul 31.

Authors

Fiona Senchyna¹, Fiona B Tamburini², Kanagavel Murugesan¹, Nancy Watz³, Ami S Bhatt², Niaz Banaei⁴

Affiliations

¹ Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA.
² Division of Hematology, Department of Medicine and Department of Genetics, Stanford University School of Medicine, Stanford, CA, USA.
³ Clinical Microbiology Laboratory, Stanford University Medical Center, Palo Alto, CA, USA.
⁴ Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA; Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA; Clinical Microbiology Laboratory, Stanford University Medical Center, Palo Alto, CA, USA. Electronic address: [email protected].

PMID: 34418822
DOI: 10.1016/j.diagmicrobio.2021.115511

Abstract

Resistance to Ceftazidime-Avibactam in Enterobacter cloacae is poorly understood. Whole genome sequencing identified 6 variants in isolates collected from a patient before and after acquiring Ceftazidime-Avibactam resistance. This included a Phe396Leu mutation in acrB, a component of the AcrAB-TolC efflux pump, possibly mediating enhanced efflux of Ceftazidime and/ or Avibactam.

Keywords: Ceftazidime-Avibactam resistance; Enterobacter cloacae; efflux pumps; whole genome sequencing.

Publication types

Comparative Study

MeSH terms

Anti-Bacterial Agents / pharmacology
Anti-Bacterial Agents / therapeutic use
Azabicyclo Compounds / pharmacology
Azabicyclo Compounds / therapeutic use*
Bacterial Proteins / genetics
Ceftazidime / pharmacology
Ceftazidime / therapeutic use*
Drug Combinations
Drug Resistance, Multiple, Bacterial / drug effects
Drug Resistance, Multiple, Bacterial / genetics*
Enterobacter cloacae / drug effects
Enterobacter cloacae / genetics*
Enterobacter cloacae / isolation & purification
Enterobacteriaceae Infections / drug therapy
Enterobacteriaceae Infections / microbiology*
Genome, Bacterial / genetics
Genomics
Humans
Male
Membrane Transport Proteins / genetics
Microbial Sensitivity Tests
Middle Aged
Mutation

Substances

Anti-Bacterial Agents
Azabicyclo Compounds
Bacterial Proteins
Drug Combinations
Membrane Transport Proteins
avibactam, ceftazidime drug combination
Ceftazidime