Bacterial cellulose (BC) has a huge global market due to its excellent properties and wide range of applications. However, due to high production costs, low productivity, and unsatisfactory scale-up production, industrialisation has been slow. Herein, stabilization of strain, optimisation of culture conditions, and a cheap carbon source were combined to achieve highly efficient, low-cost, large-scale BC production in 20 L containers. Optimisation of culture conditions increased both BC productivity and sugar conversion ratio significantly, from 2.08 g/L/day and 9.78% to 17.13 g/L/day and 70.31%, respectively. Furthermore, BC productivity and sugar conversion ratio reached 13.96 g/L/day and 85.50% using corn stover total hydrolysate as carbon source. The low-cost, facile, and highly efficient process can generate large quantities of BC, and could promote industrialisation of BC production.
Keywords: Bacterial cellulose; Cheap carbon source; Industrialisation; Productivity; Scale-up.
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