Impact of fedratinib on the pharmacokinetics of transporter probe substrates using a cocktail approach

Cancer Chemother Pharmacol. 2021 Dec;88(6):941-952. doi: 10.1007/s00280-021-04346-7. Epub 2021 Sep 3.

Abstract

Introduction: Fedratinib, an oral, selective Janus kinase 2 inhibitor, has been shown to inhibit P-glycoprotein (P-gp), breast cancer resistance protein (BCRP), organic anion transporting polypeptide (OATP) 1B1, OATP1B3, organic cation transporter (OCT) 2, and multidrug and toxin extrusion (MATE) 1 and MATE2-K in vitro. The objective of this study was to evaluate the influence of fedratinib on the pharmacokinetics (PK) of digoxin (P-gp substrate), rosuvastatin (OATP1B1/1B3 and BCRP substrate), and metformin (OCT2 and MATE1/2-K substrate).

Methods: In this nonrandomized, fixed-sequence, open-label study, 24 healthy adult participants received single oral doses of digoxin 0.25 mg, rosuvastatin 10 mg, and metformin 1000 mg administered as a drug cocktail (day 1, period 1). After a 6-day washout, participants received oral fedratinib 600 mg 1 h before the cocktail on day 7 (period 2). An oral glucose tolerance test (OGTT) was performed to determine possible influences of fedratinib on the antihyperglycemic effect of metformin.

Results: Plasma exposure to the three probe drugs was generally comparable in the presence or absence of fedratinib. Reduced metformin renal clearance by 36% and slightly higher plasma glucose levels after OGTT were observed in the presence of fedratinib. Single oral doses of the cocktail ± fedratinib were generally well tolerated.

Conclusions: These results suggest that fedratinib has minimal impact on the exposure of P-gp, BCRP, OATP1B1/1B3, OCT2, and MATE1/2-K substrates. Since renal clearance of metformin was decreased in the presence of fedratinib, caution should be exercised in using coadministered drugs that are renally excreted via OCT2 and MATEs.

Trial registration: Clinicaltrials.gov NCT04231435 on January 18, 2020.

Keywords: Digoxin; Drug–drug interaction; Fedratinib; Metformin; Rosuvastatin; Transporter.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP Binding Cassette Transporter, Subfamily B, Member 1 / antagonists & inhibitors
  • ATP Binding Cassette Transporter, Subfamily B, Member 1 / metabolism
  • ATP Binding Cassette Transporter, Subfamily G, Member 2 / antagonists & inhibitors
  • ATP Binding Cassette Transporter, Subfamily G, Member 2 / metabolism
  • Administration, Oral
  • Adolescent
  • Adult
  • Aged
  • Anticholesteremic Agents / pharmacokinetics
  • Biological Transport
  • Cardiotonic Agents / pharmacokinetics
  • Case-Control Studies
  • Digoxin / pharmacokinetics*
  • Drug Interactions*
  • Female
  • Follow-Up Studies
  • Healthy Volunteers
  • Humans
  • Hypoglycemic Agents / pharmacokinetics
  • Male
  • Metformin / pharmacokinetics*
  • Middle Aged
  • Neoplasm Proteins / antagonists & inhibitors
  • Neoplasm Proteins / metabolism
  • Non-Randomized Controlled Trials as Topic
  • Organic Anion Transporters / antagonists & inhibitors
  • Organic Anion Transporters / metabolism
  • Pyrrolidines / pharmacology*
  • Rosuvastatin Calcium / pharmacokinetics*
  • Sulfonamides / pharmacology*
  • Tissue Distribution
  • Young Adult

Substances

  • ABCG2 protein, human
  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • ATP Binding Cassette Transporter, Subfamily G, Member 2
  • Anticholesteremic Agents
  • Cardiotonic Agents
  • Hypoglycemic Agents
  • Neoplasm Proteins
  • Organic Anion Transporters
  • Pyrrolidines
  • Sulfonamides
  • fedratinib
  • Digoxin
  • Rosuvastatin Calcium
  • Metformin

Associated data

  • ClinicalTrials.gov/NCT04231435