Analytical method validation for biomarkers as a drug development tool: points to consider

Bioanalysis. 2021 Sep;13(18):1379-1389. doi: 10.4155/bio-2021-0173. Epub 2021 Sep 14.

Abstract

Biomarkers are an important drug developmental tool. Assessment of quantitative analytical methods of biomarkers is not included in any regulatory documents in Japan. Use of biomarkers in clinical evaluations and supporting the post-marketing evaluation of drug efficacy and/or adverse reactions requires assessment and full validation of analytical methods for these biomarkers. The Biomarker Analytical Method Validation Study Group is a research group in Japan comprising industry and regulatory experts. Group members discussed and prepared this 'points to consider document' covering measurements of endogenous metabolites/peptides/proteins by ligand binding assays and chromatographic methods with or without mass spectrometry. We hope this document contributes to the global harmonization of biomarker assay validation.

Keywords: Japan; assay validation; biomarker; regulatory submission; study sample analysis; white paper.

MeSH terms

  • Biomarkers / metabolism*
  • Drug Development / methods*
  • Humans

Substances

  • Biomarkers