Imaging the Actin Cytoskeleton in Fixed Budding Yeast Cells

Cierra N Sing; Emily J Yang; Ryo Higuchi-Sanabria; Liza A Pon; Istvan R Boldogh; Theresa C Swayne

doi:10.1007/978-1-0716-1661-1_4

Imaging the Actin Cytoskeleton in Fixed Budding Yeast Cells

Methods Mol Biol. 2022:2364:81-100. doi: 10.1007/978-1-0716-1661-1_4.

Authors

Cierra N Sing¹, Emily J Yang¹, Ryo Higuchi-Sanabria², Liza A Pon^{3

4}, Istvan R Boldogh^{1

5}, Theresa C Swayne⁵

Affiliations

¹ Department of Pathology and Cell Biology, Columbia University, College of Physicians and Surgeons, New York, NY, USA.
² Leonard Davis School of Gerontology, University of Southern California, Los Angeles, CA, USA.
³ Confocal and Specialized Microscopy Shared Resource, Herbert Irving Comprehensive Cancer Center, Columbia University, New York, NY, USA. [email protected].
⁴ Department of Pathology and Cell Biology, Columbia University, New York, NY, USA. [email protected].
⁵ Confocal and Specialized Microscopy Shared Resource, Herbert Irving Comprehensive Cancer Center, Columbia University, New York, NY, USA.

Abstract

Budding yeast, Saccharomyces cerevisiae, is an appealing model organism to study the organization and function of the actin cytoskeleton. With the advent of techniques to perform high-resolution, multidimensional analysis of the yeast cell, imaging of yeast has emerged as an important tool for research on the cytoskeleton. This chapter describes techniques and approaches for visualizing the actin cytoskeleton in fixed yeast cells with wide-field and super-resolution fluorescence microscopy.

Keywords: Actin; Cytoskeleton; Fluorescent proteins; Microscopy; Super-resolution structured illumination microscopy (SR-SIM); Vital staining; Yeast.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Actin Cytoskeleton
Actins
Cytoskeleton
Microscopy, Fluorescence
Saccharomyces cerevisiae*

Substances

Actins

Abstract

Publication types

MeSH terms

Substances

Grants and funding