Studying Hedgehog Signaling During Mouse Neural Tube Development

Sandii Constable; Kevin White; Bandarigoda Nipunika Somatilaka; Saikat Mukhopadhyay

doi:10.1007/978-1-0716-1701-4_6

Studying Hedgehog Signaling During Mouse Neural Tube Development

Methods Mol Biol. 2022:2374:59-71. doi: 10.1007/978-1-0716-1701-4_6.

Authors

Sandii Constable¹, Kevin White², Bandarigoda Nipunika Somatilaka^{2

3}, Saikat Mukhopadhyay⁴

Affiliations

¹ Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA. [email protected].
² Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA.
³ Department of Dermatology, University of Texas Southwestern Medical Center, Dallas, TX, USA.
⁴ Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA. [email protected].

PMID: 34562243
DOI: 10.1007/978-1-0716-1701-4_6

Abstract

The identity of ventral neural progenitors in the neural tube is largely dependent on Hedgehog (Hh) signaling. Variations in staining patterns are excellent indicators of aberrant Hh signaling. Here we describe the basic protocol to stain for progenitor populations based on transcription factor expression. We also provide an overview of ciliary and centrosomal staining in the neural tube.

Keywords: Centrosome; Cilia; Hedgehog; Immunostaining; Neural tube.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cilia
Hedgehog Proteins
Mice
Neural Tube*
Organogenesis
Signal Transduction

Substances

Hedgehog Proteins

Abstract

Publication types

MeSH terms

Substances

Grants and funding