Human tumor cells synthesize an endothelial cell growth factor that is structurally related to basic fibroblast growth factor

Proc Natl Acad Sci U S A. 1986 Apr;83(8):2448-52. doi: 10.1073/pnas.83.8.2448.

Abstract

A human hepatoma cell line synthesizes, as evidenced by metabolic labeling, an endothelial cell mitogen that is found to be mostly cell associated. The hepatoma-derived growth factor (HDGF) has been purified to homogeneity by a combination of Bio-Rex 70, heparin-Sepharose, and reverse-phase chromatography; it is a cationic polypeptide with a molecular weight of about 18,500-19,000. HDGF is structurally related to basic fibroblast growth factor (FGF). Immunological analysis demonstrates that antiserum prepared against a synthetic peptide corresponding to the amino-terminal sequence of basic FGF cross-reacts with HDGF when analyzed by electrophoretic blotting and by immunoprecipitation. Sequence analysis of tryptic fragments demonstrates that HDGF contains sequences that are homologous to both amino-terminal and carboxyl-terminal sequences of basic FGF.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Angiogenesis Inducing Agents / biosynthesis
  • Angiogenesis Inducing Agents / immunology
  • Animals
  • Cross Reactions
  • Endothelium / cytology
  • Fibroblast Growth Factors* / immunology
  • Growth Substances / biosynthesis*
  • Growth Substances / immunology
  • Humans
  • Intercellular Signaling Peptides and Proteins*
  • Liver Neoplasms, Experimental / physiopathology*
  • Peptide Fragments / analysis
  • Pituitary Gland / physiology

Substances

  • Angiogenesis Inducing Agents
  • Growth Substances
  • Intercellular Signaling Peptides and Proteins
  • Peptide Fragments
  • hepatoma-derived growth factor
  • Fibroblast Growth Factors