Effects and Mechanism of Zishen Jiangtang Pill on Diabetic Osteoporosis Rats Based on Proteomic Analysis

Shufang Chu; Deliang Liu; Hengxia Zhao; Mumin Shao; Xuemei Liu; Xin Qu; Zengying Li; Jinhua Li; Huilin Li

doi:10.1155/2021/7383062

Effects and Mechanism of Zishen Jiangtang Pill on Diabetic Osteoporosis Rats Based on Proteomic Analysis

Evid Based Complement Alternat Med. 2021 Sep 24:2021:7383062. doi: 10.1155/2021/7383062. eCollection 2021.

Authors

Shufang Chu¹, Deliang Liu¹, Hengxia Zhao¹, Mumin Shao², Xuemei Liu¹, Xin Qu¹, Zengying Li¹, Jinhua Li¹, Huilin Li¹

Affiliations

¹ Department of Endocrinology, Shenzhen TCM Hospital, The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen 518033, China.
² Department of Pathology, Shenzhen TCM Hospital, The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen 518033, China.

Abstract

Objective: To explore the effect and mechanism of ZJP on DOP rats by proteomic analysis.

Materials and methods: After the establishment of diabetes model by Streptozocin (STZ, 60 mg/kg), 40 Wistar rats were equally divided into normal group, model group (diabetic rats), high-dose group (3.0 g/kg/d ZJP), and low-dose group (1.5 g/kg/d ZJP) and received treatment for 3 months. Histological changes in bone and pancreas tissues were observed by hematoxylin and eosin staining, electron microscopy, and immunofluorescence. Proteomic and bioinformatic analyses were performed to identify the differentially expressed proteins. The fingerprint and active ingredients of ZJP were identified via high-performance liquid chromatography (HPLC).

Results: Compared with the model group, ZJP could rescue the weight, fasting blood glucose, and fasting insulin of rats in both high-dose and low-dose group. ZJP could also improve the microstructures of pancreatic islet cells, bone mass, and trabecular and marrow cavities in DOP rats. Bioinformatic analysis suggested that ZJP might influence DOP via multiple pathways, mainly including ribosomes, vitamin digestion and absorption, and fat digestion and absorption. The primary active ingredients, including notoginsenoside R1, ginsenoside Rg1, ginsenoside Re, icariin, and ginsenoside Rb1, were detected.

Conclusion: ZJP could significantly improve the histomorphology and ultrastructure of bone and islets tissues and might serve as an effective alternative medicine for the treatment of DOP.