Molecular Genetic Techniques for the Proteoglycan Functions in Drosophila

Nanako Bowden; Masahiko Takemura; Hiroshi Nakato

doi:10.1007/978-1-0716-1398-6_32

Molecular Genetic Techniques for the Proteoglycan Functions in Drosophila

Methods Mol Biol. 2022:2303:405-414. doi: 10.1007/978-1-0716-1398-6_32.

Authors

Nanako Bowden¹, Masahiko Takemura¹, Hiroshi Nakato²

Affiliations

¹ Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN, USA.
² Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN, USA. [email protected].

Abstract

Several classes of heparan sulfate proteoglycan (HSPG) core proteins and all HS biosynthetic/modifying enzymes are evolutionarily conserved from human to Drosophila melanogaster. This genetically tractable model offers highly sophisticated techniques to manipulate gene function in a spatially and temporally controlled manner. Thus, Drosophila genetics has been a powerful system to explore functions of HSPGs in vivo. In this chapter, we will introduce three genetic techniques available in Drosophila: TARGET (temporal and regional gene expression targeting), MARCM (mosaic analysis with a repressible cell marker), and FLP-Out.

Keywords: Drosophila melanogaster; FLP-Out; GAL4/UAS; HSPG; MARCM; TARGET.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Animals
Drosophila melanogaster* / genetics
Genetic Techniques
Heparan Sulfate Proteoglycans / genetics
Phenotype

Substances

Heparan Sulfate Proteoglycans

Grants and funding

R35 GM131688/GM/NIGMS NIH HHS/United States