Infecting human hematopoietic stem and progenitor cells with SARS-CoV-2

Hector Huerga Encabo; Rachel Ulferts; Aneesh Sharma; Rupert Beale; Dominique Bonnet

doi:10.1016/j.xpro.2021.100903

Infecting human hematopoietic stem and progenitor cells with SARS-CoV-2

STAR Protoc. 2021 Dec 17;2(4):100903. doi: 10.1016/j.xpro.2021.100903. Epub 2021 Oct 6.

Authors

Hector Huerga Encabo¹, Rachel Ulferts², Aneesh Sharma¹, Rupert Beale², Dominique Bonnet¹

Affiliations

¹ Hematopoietic Stem Cell Laboratory, The Francis Crick Institute, London, NW1 1AT, UK.
² Cell Biology of Infection Laboratory, The Francis Crick Institute, London NW1 1AT, UK.

Abstract

Determining how hematopoietic stem and progenitor cells (HSPCs) can be infected by viruses is necessary to understand and predict how the immune system will drive the host response. We present here a protocol to analyze the capacity of SARS-CoV-2 to infect different subsets of human HSPCs, inlcuding procedures for SARS-CoV-2 production and titration, isolation of human HSPCs from different sources (bone marrow, umbilical cord, or peripheral blood), and quantification of SARS-Cov-2 infection capacity by RT-qPCR and colony forming unit assay. For complete details on the use and execution of this protocol, please refer to Huerga Encabo et al. (2021).

Keywords: Cell Biology; Cell isolation; Cell-based Assays; Immunology; Microbiology; Microscopy; Stem Cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bone Marrow / virology*
COVID-19 / pathology
COVID-19 / virology*
COVID-19 Nucleic Acid Testing / methods*
Colony-Forming Units Assay / methods*
Fetal Blood / virology*
Hematopoietic Stem Cells / pathology
Hematopoietic Stem Cells / virology*
Humans
SARS-CoV-2 / isolation & purification*

Abstract

Publication types

MeSH terms

Grants and funding