Generation of an orthotopic mouse model to study colorectal cancer metastasis

Liwen Zhang; Pengcheng Bu

doi:10.1016/j.xpro.2021.100792

Generation of an orthotopic mouse model to study colorectal cancer metastasis

STAR Protoc. 2021 Sep 27;2(4):100792. doi: 10.1016/j.xpro.2021.100792. eCollection 2021 Dec 17.

Authors

Liwen Zhang^{1

2}, Pengcheng Bu^{1

2

3}

Affiliations

¹ Key Laboratory of RNA Biology, Key Laboratory of Protein and Peptide Pharmaceutical, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
² College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China.
³ Center for Excellence in Biomacromolecules, Chinese Academy of Sciences, Beijing 100101, China.

Abstract

This protocol describes the generation of a mouse colorectal cancer (CRC) model to study the mechanism of CRC growth and metastasis. Compared to existing protocols, this protocol is mainly to improve the incidence of metastasis. The major advantage of this model is that it mimics the process of clinical CRC metastasis. Thus, it can be used to study different stages of CRC progression and to identify molecular markers or therapeutic targets. The limitation is the difficulty of performing the operation. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2021).

Keywords: Cancer; Model Organisms.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Colonic Neoplasms / pathology*
HCT116 Cells
Humans
Mice
Neoplasm Metastasis / pathology*
Neoplasms, Experimental*