Comprehensive phenotyping of human peripheral blood B lymphocytes in healthy conditions

Rita Carsetti; Sara Terreri; Maria Giulia Conti; Ane Fernandez Salinas; Francesco Corrente; Claudia Capponi; Christian Albano; Eva Piano Mortari

doi:10.1002/cyto.a.24507

Comprehensive phenotyping of human peripheral blood B lymphocytes in healthy conditions

Cytometry A. 2022 Feb;101(2):131-139. doi: 10.1002/cyto.a.24507. Epub 2021 Oct 18.

Authors

Rita Carsetti^{1

2}, Sara Terreri¹, Maria Giulia Conti^{3

4}, Ane Fernandez Salinas^{1

4}, Francesco Corrente², Claudia Capponi², Christian Albano¹, Eva Piano Mortari¹

Affiliations

¹ Diagnostic Immunology Research Unit, Multimodal Medicine Research Area, Bambino Gesù Children's Hospital, IRCCS, Rome, Italy.
² Microbiology and Diagnostic Immunology Unit, Bambino Gesù Children's Hospital, IRCCS, Rome, Italy.
³ Department of Maternal and Child Health, Policlinico Umberto I, Sapienza University of Rome, Rome, Italy.
⁴ Department of Molecular Medicine, Sapienza University of Rome, Rome, Italy.

Abstract

The B cell compartment provides innate and adaptive immune defenses against pathogens. Different B cell subsets, reflecting the maturation stages of B cells, have noninterchangeable functions and roles in innate and adaptive immune responses. In this review, we provide an overview of the B cell subsets present in peripheral blood of healthy individuals. A specific gating strategy is also described to clearly and univocally identify B cell subsets based on the their phenotypic traits by flow cytometric analysis.

Keywords: B cell subsets; flow cytometry; human B cells; phenotyping.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

B-Lymphocytes*
Flow Cytometry
Humans
Phenotype