Clinical, Virologic and Immunologic Correlates of Breast Milk Acquired Cytomegalovirus (CMV) Infections in Very Low Birth Weight (VLBW) Infants in a Newborn Intensive Care Unit (NICU) Setting

Viruses. 2021 Sep 22;13(10):1897. doi: 10.3390/v13101897.

Abstract

Cytomegalovirus (CMV) infections acquired by very-low-birthweight (VLBW) infants are incompletely characterized. To examine CMV transmission in VLBW infants, we evaluated maternal DNAlactia, infant DNAemia, and presence of clinical disease in a blinded study in VLBW infants in our newborn intensive care unit (NICU). To examine these issues, 200 VLBW infants were enrolled in a surveillance study, with weekly breast milk and infant whole blood samples collected, as available. Virologic (breast milk and infant whole blood real time PCR) and immunologic (IgG, IgM, and IgG avidity) correlates were evaluated. A chart review examined whether infants had symptoms compatible with CMV disease. DNAlactia was identified in 65/150 (43%) of lactating mothers. Nine CMV infections were identified in 9/75 CMV-exposed infants (12% of exposed infants). A higher median breast milk viral load (DNAlactia) correlated with an increased likelihood of DNAemia (p = 0.05). Despite potential symptoms compatible with CMV infection, clinicians had not considered the diagnosis of CMV in 6/9 cases (66%). All of these infants had chronic lung disease at discharge. There was no correlation between IgG antibody titer or IgG avidity index and the likelihood of transmission or CMV disease. In conclusion, in VLBW infants receiving milk from seropositive mothers, CMV infections are commonly acquired, and are frequently unrecognized. Future studies are needed to determine whether routine surveillance for CMV of either breast milk or infant plasma is beneficial in preventing or recognizing infection.

Keywords: CMV antivirals; breast milk; cytomegalovirus (CMV); post-natal CMV.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cytomegalovirus / immunology
  • Cytomegalovirus Infections
  • DNA, Viral / analysis
  • Female
  • Humans
  • Immunoglobulin G / immunology
  • Immunoglobulin G / metabolism
  • Infant, Newborn
  • Infant, Very Low Birth Weight / immunology*
  • Infectious Disease Transmission, Vertical
  • Intensive Care Units, Neonatal / statistics & numerical data
  • Lactation
  • Milk, Human / immunology*
  • Milk, Human / virology*
  • Mothers
  • Pregnancy
  • Pregnancy Complications, Infectious / virology
  • Retrospective Studies
  • Viral Load

Substances

  • DNA, Viral
  • Immunoglobulin G