Laboratory work is an essential part of natural science education because it provides students with a valuable opportunity to experience practical scientific research firsthand. In laboratory work in pharmacology, students generally learn about biological mechanisms and drug action mechanisms by analyzing drug actions using laboratory animals. Actual experience with hands and eyes is an important factor in the laboratory work. Under the COVID-19 epidemic, however, we were forced to conduct the laboratory work online. For the laboratory work using isolated organs, we used simulation software, in which students can examine effects of a range of drugs on the smooth muscle within the guinea pig ileum. For the behavioral observation practice, we showed the video of the experiments conducted by the instructors beforehand to the students, and asked them to observe and analyze the behavior. In this review, we will share our challenges to online laboratory work.