Background: So far, few have concerned miR-497-5p in lung squamous cell carcinoma (LUSC).
Methods: MiR-497-5p expression in LUSC was measured by qRT-PCR. Its impacts on tumor-related cell behaviors were investigated by CCK8 assay, scratch healing assay, flow cytometry and Transwell invasion methods. In addition, interaction between miR-497-5p and CDCA4 in LUSC was also elucidated through rescue experiment, western blot, dual-luciferase, and bioinformatics analysis.
Results: Low level of miR-497-5p was confirmed in LUSC tissue and cells. Overexpressed miR-497-5p markedly inhibited cancer progression. miR-497-5p restrained CDCA4 expression. Rescue assay showed that overexpressing miR-497-5p eliminated effect of overexpressed CDCA4.
Conclusion: By targeting CDCA4, miR-497-5p restrained development of LUSC.
Keywords: CDCA4; Invasion; LUSC; Migration; Proliferation; miR-497-5p.
© 2021. The Author(s).