qPrimerDB: A Powerful and User-Friendly Database for qPCR Primer Design

Wei Chang; Yue Niu; Mengna Yu; Tian Li; Jiana Li; Kun Lu

doi:10.1007/978-1-0716-1799-1_13

qPrimerDB: A Powerful and User-Friendly Database for qPCR Primer Design

Methods Mol Biol. 2022:2392:173-182. doi: 10.1007/978-1-0716-1799-1_13.

Authors

Wei Chang¹, Yue Niu¹, Mengna Yu¹, Tian Li^{2

3}, Jiana Li^{1

4

5}, Kun Lu^{6

7

8}

Affiliations

¹ College of Agronomy and Biotechnology, Southwest University, Chongqing, China.
² State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
³ Chongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing, China.
⁴ Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing, China.
⁵ Academy of Agricultural Sciences, Southwest University, Chongqing, China.
⁶ College of Agronomy and Biotechnology, Southwest University, Chongqing, China. [email protected].
⁷ Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing, China. [email protected].
⁸ Academy of Agricultural Sciences, Southwest University, Chongqing, China. [email protected].

PMID: 34773623
DOI: 10.1007/978-1-0716-1799-1_13

Abstract

Real-time quantitative polymerase chain reaction (qPCR) is a powerful tool for analyzing and quantifying gene expression, and its primer design is the first and most important step. In order to improve the efficiency and effectiveness of primer design, we designed a database qPrimerDB, based on thermodynamics gene-specific for multispecies qPCR primers design.In this chapter, we explained the working principle of the database and detailed the step-by-step practical steps with examples. The valuable and time-saving qPrimerDB database is publicly accessible at http://biodb.swu.edu.cn/qprimerdb and will be routinely updated.

Keywords: Free online database; High-level efficiency; Primer design; Quantitative real-time polymerase chain reaction; qPrimerDB.

MeSH terms

DNA Primers / genetics
Databases, Genetic
Gene Expression
Real-Time Polymerase Chain Reaction*
Thermodynamics

Substances

DNA Primers