Multiple stages of evolutionary change in anthrax toxin receptor expression in humans

Nat Commun. 2021 Nov 15;12(1):6590. doi: 10.1038/s41467-021-26854-z.

Abstract

The advent of animal husbandry and hunting increased human exposure to zoonotic pathogens. To understand how a zoonotic disease may have influenced human evolution, we study changes in human expression of anthrax toxin receptor 2 (ANTXR2), which encodes a cell surface protein necessary for Bacillus anthracis virulence toxins to cause anthrax disease. In immune cells, ANTXR2 is 8-fold down-regulated in all available human samples compared to non-human primates, indicating regulatory changes early in the evolution of modern humans. We also observe multiple genetic signatures consistent with recent positive selection driving a European-specific decrease in ANTXR2 expression in multiple tissues affected by anthrax toxins. Our observations fit a model in which humans adapted to anthrax disease following early ecological changes associated with hunting and scavenging, as well as a second period of adaptation after the rise of modern agriculture.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Antigens, Bacterial
  • Bacillus anthracis / genetics
  • Bacterial Toxins
  • Cell Line
  • Down-Regulation
  • Evolution, Molecular*
  • Gene Expression Regulation*
  • Humans
  • K562 Cells
  • Membrane Proteins / metabolism
  • Receptors, Peptide / genetics*
  • Receptors, Peptide / metabolism*
  • Virulence
  • Zoonoses

Substances

  • ANTXR2 protein, human
  • Antigens, Bacterial
  • Bacterial Toxins
  • Membrane Proteins
  • Receptors, Peptide
  • anthrax toxin
  • anthrax toxin receptors