Analysing microbiome intervention design studies: Comparison of alternative multivariate statistical methods

PLoS One. 2021 Nov 18;16(11):e0259973. doi: 10.1371/journal.pone.0259973. eCollection 2021.

Abstract

The diet plays a major role in shaping gut microbiome composition and function in both humans and animals, and dietary intervention trials are often used to investigate and understand these effects. A plethora of statistical methods for analysing the differential abundance of microbial taxa exists, and new methods are constantly being developed, but there is a lack of benchmarking studies and clear consensus on the best multivariate statistical practices. This makes it hard for a biologist to decide which method to use. We compared the outcomes of generic multivariate ANOVA (ASCA and FFMANOVA) against statistical methods commonly used for community analyses (PERMANOVA and SIMPER) and methods designed for analysis of count data from high-throughput sequencing experiments (ALDEx2, ANCOM and DESeq2). The comparison is based on both simulated data and five published dietary intervention trials representing different subjects and study designs. We found that the methods testing differences at the community level were in agreement regarding both effect size and statistical significance. However, the methods that provided ranking and identification of differentially abundant operational taxonomic units (OTUs) gave incongruent results, implying that the choice of method is likely to influence the biological interpretations. The generic multivariate ANOVA tools have the flexibility needed for analysing multifactorial experiments and provide outputs at both the community and OTU levels; good performance in the simulation studies suggests that these statistical tools are also suitable for microbiome data sets.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Computer Simulation
  • Datasets as Topic
  • Diet
  • Gastrointestinal Microbiome*
  • High-Throughput Nucleotide Sequencing
  • Humans
  • Multivariate Analysis*

Grants and funding

This work was funded by Nofima – Norwegian Institute of Food, Fisheries and Aquaculture Research and Foundation for Research Levy on Agricultural Products (Research Council of Norway projects No. 262306, 262308, 314111 and 314743). The funding sponsors had no role in the design of the study, the collection, analyses and interpretation of data, in the writing of the manuscript or in the decision to distribute the results.