Antimicrobial Effect of Calcium Hydroxide Combined with Electrolyzed Superoxidized Solution at Neutral pH on Enterococcus faecalis Growth

Biomed Res Int. 2021 Nov 9:2021:6960143. doi: 10.1155/2021/6960143. eCollection 2021.

Abstract

Objective: To evaluate the effect of the combination of calcium hydroxide (Ca(OH)2) and a novel electrolyzed superoxidized solution at neutral pH, known as OxOral® on Enterococcus faecalis growth in root canals.

Methods: Sixty human teeth were used, from which root canals were infected and randomly divided into the following treatment groups: saline solution, saline solution plus Ca(OH)2, OxOral®, and OxOral® plus Ca(OH)2.

Results: A permanent reduction in bacterial growth was observed at days 1, 6, 12, and 18 after OxOral® plus Ca(OH)2 treatment from 4.4 ± 0.074 log10 CFU/mL to 0.0 ± 0.001 log10 CFU/mL. In addition, alkaline conditions maintenance was observed from application time (pH = 12.2 ± 0.033) to 18 d posttreatment (pH = 12.6 ± 0.083).

Conclusion: The combination of OxOral® and Ca(OH)2 provides an alkaline pH and inhibits E. faecalis growth into the root canals. Our study opens the possibility for further research on the use of OxOral® in endodontic therapy.

MeSH terms

  • Anti-Infective Agents / administration & dosage*
  • Calcium Hydroxide / administration & dosage*
  • Dental Pulp Cavity / drug effects*
  • Dental Pulp Cavity / microbiology*
  • Enterococcus faecalis / drug effects*
  • Enterococcus faecalis / growth & development
  • Humans
  • Hydrogen Peroxide / administration & dosage*
  • Hydrogen Peroxide / chemistry
  • Hydrogen-Ion Concentration
  • In Vitro Techniques
  • Root Canal Irrigants / administration & dosage
  • Root Canal Irrigants / chemistry
  • Root Canal Therapy / methods
  • Solutions

Substances

  • Anti-Infective Agents
  • Root Canal Irrigants
  • Solutions
  • superoxidized water
  • Hydrogen Peroxide
  • Calcium Hydroxide