Denitrifying bacteria immobilized magnetic mycelium pellets bioreactor: A new technology for efficient removal of nitrate at a low carbon-to-nitrogen ratio

This study integrated spores and magnetite (Fe₃O₄) to form magnetic mycelium pellets (MMP) as bio-carriers immobilized with denitrifying bacteria in a bioreactor. Different carbon-to-nitrogen (C/N) ratios and hydraulic retention time (HRT) were established for investigating the performance of the bioreactor. The nitrate removal efficiency was 98.14% at C/N = 2.0 and HRT = 6 h. Gas chromatography (GC) results indicated that the main component of the produced gas was N₂. Fe₃O₄ was well-integrated into MMP according to X-ray diffraction (XRD) results and infrared spectrometer (FTIR) analysis. Scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS) showed that bacteria were successfully immobilized on MMP. Fluorescence excitation-emission matrix (EEM) indicated that functional bacteria GF2 might enhance the metabolic activity of the microbial community in the bioreactor and microbial activity was highest at C/N = 2.0. Pseudomonas stutzeri sp. GF2 might be immobilized and had a major role in the bioreactor according to high throughput sequencing results.