H19- and hsa-miR-338-3p-mediated NRP1 expression is an independent predictor of poor prognosis in glioblastoma

PLoS One. 2021 Nov 29;16(11):e0260103. doi: 10.1371/journal.pone.0260103. eCollection 2021.

Abstract

Glioblastoma multiforme (GBM) is the most common and also the most invasive brain cancer. GBM progression is rapid and its prognosis is poor. Therefore, finding molecular targets in GBM is a critical goal that could also play important roles in clinical diagnostics and treatments to improve patient prognosis. We jointly analyzed the GSE103227, GSE103229, and TCGA databases for differentially expressed RNA species, obtaining 52 long non-coding RNAs (lncRNAs), 31 microRNAs (miRNAs), and 186 mRNAs, which were used to build a competing endogenous RNA network. Kaplan-Meier and receiver operating characteristic (ROC) analyses revealed five survival-related lncRNAs: H19, LINC01574, LINC01614, RNF144A-AS1, and OSMR-AS1. With multiple optimization mRNAs, we found the H19-hsa-miR-338-3P-NRP1 regulatory pathway. Additionally, we noted high NRP1 expression in GBM patients, and Kaplan-Meier and ROC analyses showed that NRP1 expression was associated with GBM prognosis. Cox analysis indicated that NRP1 is an independent prognostic factor in GBM patients. In conclusion, H19 and hsa-miR-338-3P regulate NRP1 expression, and this pathway plays an important role in GBM.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Brain / metabolism
  • Brain Neoplasms / genetics
  • Databases, Genetic
  • Glioblastoma / genetics
  • Glioblastoma / mortality
  • Humans
  • Kaplan-Meier Estimate
  • MicroRNAs / genetics*
  • MicroRNAs / metabolism
  • Neuropilin-1 / genetics*
  • Neuropilin-1 / metabolism
  • Prognosis
  • RNA, Long Noncoding / genetics*
  • ROC Curve

Substances

  • H19 long non-coding RNA
  • MIRN338 microRNA, human
  • MicroRNAs
  • NRP1 protein, human
  • RNA, Long Noncoding
  • Neuropilin-1

Grants and funding

The present study was supported by the National Natural Science Foundation of China (grant no. 81874081, 81672490), the Foundation of Jiangsu Provincial Health Department (grant no. YG201514), the Foundation of Xuzhou Medical University (grant no. 2018KJ09), Xuzhou Science and Technology Plan Project (grant no. KC20139), the National Demonstration Center for Experimental Basic Medical Science Education (Xuzhou Medical University), and the National Innovation and Entrepreneurship Training Program for College Students (grant no. 201910313026 and 201910313019Z).