Correlation of dried blood spots and plasma for quantification of Immunoglobulin (IgG) against Receptor binding domain and full length spike protein of SARS-CoV-2

Gaurav Kwatra; Marta Nunes; Nisha Dhar; Vicky Baillie; Natali Serafin; Stephanie Jones; Shabir A Madhi

doi:10.1016/j.jviromet.2021.114394

Correlation of dried blood spots and plasma for quantification of Immunoglobulin (IgG) against Receptor binding domain and full length spike protein of SARS-CoV-2

J Virol Methods. 2022 Feb:300:114394. doi: 10.1016/j.jviromet.2021.114394. Epub 2021 Nov 30.

Authors

Gaurav Kwatra¹, Marta Nunes², Nisha Dhar², Vicky Baillie², Natali Serafin², Stephanie Jones², Shabir A Madhi²

Affiliations

¹ South African Medical Research Council: Vaccines and Infectious Diseases Analytics Research Unit, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa; Department of Science and Technology/ National Research Foundation: Vaccine Preventable Diseases, University of the Witwatersrand, Faculty of Health Science, Johannesburg, South Africa; Department of Clinical Microbiology, Christian Medical College, Vellore, India. Electronic address: [email protected].
² South African Medical Research Council: Vaccines and Infectious Diseases Analytics Research Unit, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa; Department of Science and Technology/ National Research Foundation: Vaccine Preventable Diseases, University of the Witwatersrand, Faculty of Health Science, Johannesburg, South Africa.

Abstract

We compared plasma and dried blood spots for detection of SARS-CoV-2 IgG antibodies. There was a good correlation between IgG values measured by both sampling methods, r = 0.935 and 0.965 for Receptor Binding Domain and full-length spike protein of SARS-CoV-2. Bland-Altman assessment showed good agreement between two sampling methods. Dried blood spots is a more pragmatic method for collecting samples for sero-epidemiological surveys of SARS-CoV-2 infection.

Keywords: Dried blood spots; Full length spike protein; Immunoglobulin (IgG); Receptor binding domain; SARS-CoV-2.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Antibodies, Viral / blood*
COVID-19* / diagnosis
Dried Blood Spot Testing*
Humans
Immunoglobulin G / blood*
SARS-CoV-2
Spike Glycoprotein, Coronavirus / immunology*

Substances

Antibodies, Viral
Immunoglobulin G
Spike Glycoprotein, Coronavirus
spike protein, SARS-CoV-2