Micro-CT-Based Bone Microarchitecture Analysis of the Murine Skull

Jenny Tan; Agatha Labrinidis; Ruth Williams; Mustafa Mian; Peter J Anderson; Sarbin Ranjitkar

doi:10.1007/978-1-0716-1847-9_10

Micro-CT-Based Bone Microarchitecture Analysis of the Murine Skull

Methods Mol Biol. 2022:2403:129-145. doi: 10.1007/978-1-0716-1847-9_10.

Authors

Jenny Tan¹, Agatha Labrinidis², Ruth Williams², Mustafa Mian¹, Peter J Anderson^{1

3

4}, Sarbin Ranjitkar^{5

6}

Affiliations

¹ Adelaide Dental School, The University of Adelaide, Adelaide, SA, Australia.
² Adelaide Microscopy, The University of Adelaide, Adelaide, SA, Australia.
³ Australian Craniofacial Unit, Women's and Children's Hospital, North Adelaide, SA, Australia.
⁴ South Australian Health and Medical Research Institute (SAHMRI), Adelaide, SA, Australia.
⁵ Adelaide Dental School, The University of Adelaide, Adelaide, SA, Australia. [email protected].
⁶ Department of Dentistry and Oral Health, La Trobe Rural Health School, La Trobe University, Bendigo, VIC, Australia. [email protected].

PMID: 34913121
DOI: 10.1007/978-1-0716-1847-9_10

Abstract

X-ray micro-computed tomography (micro-CT) imaging has important applications in microarchitecture analysis of cortical and trabecular bone structure. While standardized protocols exist for micro-CT-based microarchitecture assessment of long bones, specific protocols need to be developed for different types of skull bones taking into account differences in embryogenesis, organization, development, and growth compared to the rest of the body. This chapter describes the general principles of bone microarchitecture analysis of murine craniofacial skeleton to accommodate for morphological variations in different regions of interest.

Keywords: 3D imaging; Animal model; Cortical bone; Craniofacial; Cranium; Mandible; Maxilla; Mouse; Quantitative analysis; Trabecular bone.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bone Density
Cancellous Bone
Head
Mice
Skull* / diagnostic imaging
X-Ray Microtomography