The Role of RIPC in Preventing Organ Damage, Inflammation, and Oxidative Stress during Lower Limb DSA: A Randomised Controlled Trial

Karl Kuusik; Teele Kasepalu; Mihkel Zilmer; Jaan Eha; Mare Vähi; Liisi Anette Torop; Jüri Lieberg; Jaak Kals

doi:10.1155/2021/6043550

The Role of RIPC in Preventing Organ Damage, Inflammation, and Oxidative Stress during Lower Limb DSA: A Randomised Controlled Trial

Oxid Med Cell Longev. 2021 Dec 8:2021:6043550. doi: 10.1155/2021/6043550. eCollection 2021.

Authors

Karl Kuusik^{1

2

3}, Teele Kasepalu², Mihkel Zilmer³, Jaan Eha^{1

2}, Mare Vähi⁴, Liisi Anette Torop⁵, Jüri Lieberg⁶, Jaak Kals^{3

6

7}

Affiliations

¹ Department of Cardiology, Institute of Clinical Medicine, University of Tartu, Puusepa 8, Tartu 50406, Estonia.
² Heart Clinic, Tartu University Hospital, Puusepa 8, Tartu 50406, Estonia.
³ Department of Biochemistry, Institute of Biomedicine and Translational Medicine, Centre of Excellence for Genomics and Translational Medicine, University of Tartu, Puusepa 8, Tartu 50406, Estonia.
⁴ Institute of Mathematics and Statistics, University of Tartu, J. Liivi 2, Tartu 50409, Estonia.
⁵ Pathology Service, Tartu University Hospital, Puusepa 8, Tartu 50406, Estonia.
⁶ Department of Vascular Surgery, Surgery Clinic, Tartu University Hospital, Puusepa 8, Tartu 50406, Estonia.
⁷ Department of Surgery, Institute of Clinical Medicine, University of Tartu, Puusepa 8, Tartu 50406, Estonia.

Abstract

Objective: Diagnostic digital subtraction angiography (DSA) and DSA with percutaneous transluminal angioplasty (DSA-PTA) are common procedures for diagnosing and treating symptomatic lower extremity arterial disease (LEAD). However, organ damage following DSA and DSA-PTA is often underrecognised and hence undiagnosed. To reduce the risk induced by invasive procedures in symptomatic LEAD patients, the method of remote ischemic preconditioning (RIPC) has been suggested. The aim of the current study was to assess the effect of RIPC intervention on the organ damage markers profile, oxidative stress, and inflammation biomarkers in LEAD patients undergoing DSA and DSA-PTA procedure.

Methods: The RIPC intervention was performed by inflating a standard blood pressure cuff on the patient's upper arm to 200 mmHg for 5 minutes four times with 5-minute perfusion between each cycle. The sham intervention was performed similarly, but the cuff was inflated to 20 mmHg. Changes in the cardiac and renal damage biomarkers' profile, oxidative stress, and inflammation biomarkers were recorded before and 24 hours after DSA or DSA-PTA.

Results: A total of 111 (RIPC 54, sham 57) patients with symptomatic LEAD scheduled for endovascular procedure were randomised, and 102 patients (RIPC 47, sham 55) completed the study protocol. RIPC significantly limited the increase of adiponectine levels after DSA and DSA-PTA, compared to sham intervention (p = 0.020), but CK-MB levels were markedly lower in the sham group (p = 0.047) after procedure. There was no significant difference between the RIPC and the sham group in mean changes in hs-troponin-T (p = 0.25), NT-proBNP (p = 0.24), creatinine (p = 0.76), eGFR (p = 0.61), urea (p = 0.95), beta-2-microglobuline (p = 0.34), or cystatine C (p = 0.24) levels.

Conclusion: In this controlled clinical study, RIPC failed to improve the profile of renal and cardiac biomarkers in patients with LEAD periprocedurally. RIPC significantly limits the rise in adiponectin levels and may influence the decrease of CK-MB levels 24 hours after endovascular procedure.

Publication types

Randomized Controlled Trial

MeSH terms

Aged
Angiography, Digital Subtraction / methods*
Case-Control Studies
Double-Blind Method
Female
Humans
Inflammation / prevention & control*
Ischemic Preconditioning / methods*
Lower Extremity / blood supply*
Male
Multiple Organ Failure / prevention & control*
Oxidative Stress*
Peripheral Arterial Disease / pathology
Peripheral Arterial Disease / therapy*