Ultrasmall Au nanoparticles modified 2D metalloporphyrinic metal-organic framework nanosheets with high peroxidase-like activity for colorimetric detection of organophosphorus pesticides

Huanyu Yang; Zhiping Sun; Xinguang Qin; Hongyuan Wu; Haizhi Zhang; Gang Liu

doi:10.1016/j.foodchem.2021.131906

Ultrasmall Au nanoparticles modified 2D metalloporphyrinic metal-organic framework nanosheets with high peroxidase-like activity for colorimetric detection of organophosphorus pesticides

Food Chem. 2021 Dec 22:376:131906. doi: 10.1016/j.foodchem.2021.131906. Online ahead of print.

Authors

Huanyu Yang¹, Zhiping Sun¹, Xinguang Qin¹, Hongyuan Wu¹, Haizhi Zhang², Gang Liu³

Affiliations

¹ College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China.
² College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China. Electronic address: [email protected].
³ College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China. Electronic address: [email protected].

PMID: 34968912
DOI: 10.1016/j.foodchem.2021.131906

Abstract

Ultrasmall Au nanoparticles (UsAuNPs) in the size range of 4.0-7.0 nm was successfully immobilized on the surface of 2D metalloporphyrinic metal-organic framework nanosheets (2D MOF). Firstly, The obtained hybrid nanomaterial, UsAuNPs/2D MOF, was fully characterized by TEM, HRTEM, element mapping images and XPS. Then, the peroxidase-like activity of UsAuNPs/2D MOF was comparatively studied with other hybrid nanozyme to explore the influence of AuNPs size on peroxidase-like activity. Further, UsAuNPs/2D MOF with outstanding peroxidase-like activity was selected to form ternary cascade enzyme reaction with acetylcholinesterase (AChE) and choline oxidase (ChOx). Based on the inhibitory effect of organophosphorus pesticides on AChE, a fast and sensitive colorimetric method was established for trichlorfon detection with the advantages of simple operation, low detection limit (1.7 μM), good linear range (1.7-42.4 μM) and high accuracy (recovery rate of 96.6-105.3%). Finally, this method was applied to visual analysis of trichlorfon concentration in tomatoes, cucumbers and eggplants.

Keywords: Au nanoparticles; Colorimetric detection; Nanozyme; Organophosphorus pesticides; Peroxidase-like activity; Two-dimension metal-organic frameworks.