Generation of iPSC line (FAMRCi009-A) from patient with familial progressive cardiac conduction disorder carrying genetic variant FLNC p.Val2264Met

Nataliia Rodina; Aleksandr Khudiakov; Kseniya Perepelina; Aleksey Muravyev; Aleksandr Boytsov; Anna Zlotina; Polina Sokolnikova; Anna Kostareva

doi:10.1016/j.scr.2021.102640

Generation of iPSC line (FAMRCi009-A) from patient with familial progressive cardiac conduction disorder carrying genetic variant FLNC p.Val2264Met

Stem Cell Res. 2022 Mar:59:102640. doi: 10.1016/j.scr.2021.102640. Epub 2021 Dec 27.

Authors

Nataliia Rodina¹, Aleksandr Khudiakov¹, Kseniya Perepelina², Aleksey Muravyev¹, Aleksandr Boytsov³, Anna Zlotina¹, Polina Sokolnikova¹, Anna Kostareva¹

Affiliations

¹ Almazov National Medical Research Centre, Saint-Petersburg, Russia.
² Almazov National Medical Research Centre, Saint-Petersburg, Russia; Saint Petersburg State University, Saint-Petersburg, Russia. Electronic address: [email protected].
³ Almazov National Medical Research Centre, Saint-Petersburg, Russia; ITMO University, Saint-Petersburg, Russia.

PMID: 34971933
DOI: 10.1016/j.scr.2021.102640

Abstract

Human iPSC cell line FAMRCi009-A was generated from a patient with restrictive cardiomyopathy and congenital myopathy carrying FLNC p.Val2264Met genetic variant. Patient-specific peripheral blood mononuclear cells were reprogrammed using non-integrative Sendai viruses. Generated iPSC lines showed normal karyotype, expressed pluripotency markers and exhibited trilineage differentiation potential in vitro. The reported iPSC lines could be used for a deeper study of filaminopathies.