Abstract
Osteopontin is a 44 kd phosphoprotein abundant in bone matrix. We isolated a partial length cDNA for rat osteopontin and used it to examine its tissue specificity, its expression during bone development and its hormonal regulation. Osteopontin mRNA is most abundant in bone but is also found in considerable amounts in kidney. Osteopontin mRNA is regulated by the osteotropic hormones dexamethasone and 1,25(OH)2D3. Estimates of osteopontin mRNA levels indicate that the osteopontin gene is turned on relatively late in calvarial development.
MeSH terms
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Age Factors
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Alkaline Phosphatase / metabolism
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Animals
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Bone Development
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Bone Matrix / metabolism*
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Calcitriol / pharmacology
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Calcium-Binding Proteins / metabolism
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Carrier Proteins / metabolism
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Collagen / metabolism
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Dexamethasone / pharmacology
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Gene Expression Regulation / drug effects
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Kidney / physiology
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Osteoblasts / metabolism
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Osteocalcin
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Osteonectin
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Osteopontin
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RNA, Messenger / metabolism
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Rats
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Sialoglycoproteins / genetics
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Sialoglycoproteins / metabolism*
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Tissue Distribution
Substances
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Calcium-Binding Proteins
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Carrier Proteins
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Osteonectin
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RNA, Messenger
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Sialoglycoproteins
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Spp1 protein, rat
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Osteocalcin
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Osteopontin
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Dexamethasone
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Collagen
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Alkaline Phosphatase
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Calcitriol