Studies of the Formation and Stability of Ezetimibe-Cyclodextrin Inclusion Complexes

Marta Biernacka; Tatsiana Ilyich; Ilya Zavodnik; Bartłomiej Pałecz; Artur Stepniak

doi:10.3390/ijms23010455

Studies of the Formation and Stability of Ezetimibe-Cyclodextrin Inclusion Complexes

Int J Mol Sci. 2021 Dec 31;23(1):455. doi: 10.3390/ijms23010455.

Authors

Marta Biernacka¹, Tatsiana Ilyich², Ilya Zavodnik², Bartłomiej Pałecz¹, Artur Stepniak¹

Affiliations

¹ Unit of Biophysical Chemistry, Department of Physical Chemistry, Faculty of Chemistry, University of Lodz, Pomorska 163/165, 90-236 Lodz, Poland.
² Department of Biochemistry, Yanka Kupala State University of Grodno, BLK-50, 230030 Grodno, Belarus.

Abstract

In the presented studies, the interactions between ezetimibe (EZE) and selected cyclodextrins were investigated. α-Cyclodextrin (αCD), β-cyclodextrin (βCD) and its modified derivatives, hydroxypropyl-β-cyclodextrin (HPβCD) and sulfobutylether-β-cyclodextrin (SBEβCD), were selected for the research. Measurements were carried out using calorimetric and spectroscopic methods. Additionally, the Hirshfeld surface and biochemical analysis were achieved. As a result of the study, the inclusion complexes with 1:1 stoichiometry were obtained. The most stable are the complexes of β-cyclodextrin and its derivatives. The comparison of βCD with its derivatives shows that the modifications have an affect on the formation of more durable and stable complexes.

Keywords: FT-IR; HNMR; cyclodextrins; differential scanning calorimetry; formation constant; isothermal titration calorimetry; mitochondrial permeability transition.

MeSH terms

Chemistry, Pharmaceutical / methods
Cyclodextrins / chemistry*
Ezetimibe / chemistry*
Thermodynamics

Substances

Cyclodextrins
Ezetimibe