A method for fast energy estimation and visualization of protein-ligand interaction

J Comput Aided Mol Des. 1987 Oct;1(3):197-210. doi: 10.1007/BF01677044.

Abstract

A new computational and graphical method for facilitating ligand-protein docking studies is developed on a three-dimensional computer graphics display. Various physical and chemical properties inside the ligand binding pocket of a receptor protein, whose structure is elucidated by X-ray crystal analysis, are calculated on three-dimensional grid points and are stored in advance. By utilizing those tabulated data, it is possible to estimate the non-bonded and electrostatic interaction energy and the number of possible hydrogen bonds between protein and ligand molecules in real time during an interactive docking operation. The method also provides a comprehensive visualization of the local environment inside the binding pocket. With this method, it becomes easier to find a roughly stable geometry of ligand molecules, and one can therefore make a rapid survey of the binding capability of many drug candidates. The method will be useful for drug design as well as for the examination of protein-ligand interactions.

MeSH terms

  • Binding Sites
  • Computer Graphics
  • Drug Design
  • Hydrogen Bonding
  • Ligands
  • Models, Molecular*
  • Molecular Structure
  • Protein Binding
  • Protein Conformation
  • Proteins*
  • Tetrahydrofolate Dehydrogenase
  • Thermodynamics

Substances

  • Ligands
  • Proteins
  • Tetrahydrofolate Dehydrogenase