E2F transcription factor 2-activated DLEU2 contributes to prostate tumorigenesis by upregulating serum and glucocorticoid-induced protein kinase 1

Cell Death Dis. 2022 Jan 24;13(1):77. doi: 10.1038/s41419-022-04525-1.

Abstract

Long noncoding RNAs (lncRNAs) participate in biological processes in multiple types of tumors. However, the regulatory patterns of lncRNAs in prostate cancer remain largely unclear. Here, we evaluated the expression and roles of the lncRNA DLEU2 in prostate cancer. Our results showed that DLEU2 was upregulated in advanced prostate cancer tissues. Patients with prostate cancer displaying high expression of DLEU2 had a poor prognosis. Moreover, we demonstrated that overexpression of DLEU2 facilitated the proliferation, migration, and invasion of prostate cancer in vitro. Mechanistically, DLEU2 promoted serum and glucocorticoid-induced protein kinase 1 (SGK1) expression by acting as an miR-582-5p sponge, and the transcription of DLEU2 was activated by the dysregulation of E2F transcription factor 2 (E2F2) expression in prostate cancer. Furthermore, knockdown of DLEU2 attenuated prostate cancer tumorigenesis in vivo. Notably, these findings suggested that E2F2-activated DLEU2 may function as a competing endogenous RNA to facilitate prostate cancer progression by targeting the miR-582-5p/SGK1 axis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carcinogenesis / genetics
  • Cell Line, Tumor
  • Cell Movement / genetics
  • Cell Proliferation / genetics
  • Cell Transformation, Neoplastic / genetics
  • E2F2 Transcription Factor* / genetics
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Immediate-Early Proteins / metabolism
  • Male
  • MicroRNAs / genetics
  • Prostate / metabolism
  • Prostatic Neoplasms* / genetics
  • Protein Serine-Threonine Kinases / metabolism
  • RNA, Long Noncoding* / genetics

Substances

  • DLEU2 lncRNA, human
  • E2F2 Transcription Factor
  • E2F2 protein, human
  • Immediate-Early Proteins
  • MIRN582 microRNA, human
  • MicroRNAs
  • RNA, Long Noncoding
  • Protein Serine-Threonine Kinases
  • serum-glucocorticoid regulated kinase