Characterization of Lymphatic Vasculature Using Whole-Mount Immunostaining of Mouse Embryonic Dorsal Skin

Nikita Ved; Jacinta Isabelle Kalisch-Smith

doi:10.1007/978-1-0716-2059-5_6

Characterization of Lymphatic Vasculature Using Whole-Mount Immunostaining of Mouse Embryonic Dorsal Skin

Methods Mol Biol. 2022:2441:77-83. doi: 10.1007/978-1-0716-2059-5_6.

Authors

Nikita Ved¹, Jacinta Isabelle Kalisch-Smith²

Affiliations

¹ Department of Physiology, Anatomy, and Genetics, BHF Centre of Research Excellence, University of Oxford, Oxford, UK. [email protected].
² Department of Physiology, Anatomy, and Genetics, BHF Centre of Research Excellence, University of Oxford, Oxford, UK.

PMID: 35099729
DOI: 10.1007/978-1-0716-2059-5_6

Abstract

Understanding the development of the lymphatic vasculature is essential to the understanding of how these vessels function in health and disease. High-resolution imaging of histological techniques such as immunostaining of sectioned tissue provides a snapshot into lymphatic vessel morphogenesis, patterning, and organization. Whole-mount staining of embryonic dermal vasculature allows for a deeper analysis and characterization of the developing lymphatic vascular network.

Keywords: Branching; Characterization; Immunostaining; Lymphatic vessels; Vessel width.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Lymphatic System
Lymphatic Vessels*
Mice
Morphogenesis
Skin* / blood supply
Staining and Labeling

Abstract

Publication types

MeSH terms

Grants and funding