The transcriptional profile of adipose-derived stromal cells (ASC) mirrors the whitening of adipose tissue with age

Ilaria Scambi; Daniele Peroni; Alice Nodari; Flavia Merigo; Donatella Benati; Federico Boschi; Silvia Mannucci; Andrea Frontini; Silvia Visonà; Andrea Sbarbati; Mauro Krampera; Mirco Galiè

doi:10.1016/j.ejcb.2022.151206

The transcriptional profile of adipose-derived stromal cells (ASC) mirrors the whitening of adipose tissue with age

Eur J Cell Biol. 2022 Apr;101(2):151206. doi: 10.1016/j.ejcb.2022.151206. Epub 2022 Feb 8.

Authors

Affiliations

¹ Department Neuroscience, Biomedicine and Movement, Sec. Anatomy and Histology, Universitity of Verona, Verona, Italy.
² Laboratory of Translational Genomics, Centre for Integrative Biology, University of Trento, Trento, Italy.
³ Department of Computer Science, University of Verona, Strada Le Grazie 15, 37134 Verona, Italy.
⁴ Department of Life and Environmental Sciences, Polytechnic University of Marche, Ancona, Italy.
⁵ Department of Public Health Experimental and Forensic Medicine, University of Pavia, Pavia, Italy.
⁶ Department of Medicine, Section Hematology, Stem Cell Research Laboratory, University of Verona, Italy.
⁷ Department Neuroscience, Biomedicine and Movement, Sec. Anatomy and Histology, Universitity of Verona, Verona, Italy. Electronic address: [email protected].

PMID: 35158302
DOI: 10.1016/j.ejcb.2022.151206

Abstract

Multipotent stem cells persist within the stromal vascular fraction (SVF) of adipose tissue during adulthood. These cells, commonly referred to as adipose-derived stromal cells (ASC), have been extensively investigated over the past years as a promising therapeutic tool based on their regenerative and immunomodulatory properties. However, how ASC might mirror the age-related alteration of the fat they reside in remains unclear. Herein, we show that inguinal adipose tissue in mice turns from brown/beige- to white-like with age and resident ASC readily mirror these changes both at mRNA and microRNA transcriptional level. Mechanistically, our data suggest that these brown/age-related changes in ASC transcription rely on changes in the activity of E2F1 and NFkB transcription factors.

Keywords: Adipose tissue; Adipose-derived stromal cells; Aging; Beige adipocyte; White adipocyte; microRNA.

MeSH terms

Adipose Tissue*
Animals
Mice
Stromal Cells*