Superparamagnetic Iron Oxide Nanoparticles and Static Magnetic Field Regulate Neural Stem Cell Proliferation

Dan Li; Yangnan Hu; Hao Wei; Wei Chen; Yun Liu; Xiaoqian Yan; Lingna Guo; Menghui Liao; Bo Chen; Renjie Chai; Mingliang Tang

doi:10.3389/fncel.2021.815280

Superparamagnetic Iron Oxide Nanoparticles and Static Magnetic Field Regulate Neural Stem Cell Proliferation

Front Cell Neurosci. 2022 Feb 4:15:815280. doi: 10.3389/fncel.2021.815280. eCollection 2021.

Authors

Dan Li^{1

2

3}, Yangnan Hu^{2

3}, Hao Wei⁴, Wei Chen^{2

3}, Yun Liu^{2

3}, Xiaoqian Yan^{2

3}, Lingna Guo^{2

3

5}, Menghui Liao^{2

3}, Bo Chen⁶, Renjie Chai^{2

3}, Mingliang Tang^{2

7}

Affiliations

¹ School of Biology, Food and Environment, Hefei University, Hefei, China.
² Co-innovation Center of Neuroregeneration, Nantong University, Nantong, China.
³ School of Life Sciences and Technology, Southeast University, Nanjing, China.
⁴ Department of Otorhinolaryngology Head and Neck Surgery, Drum Tower Clinical Medical College, Nanjing Medical University, Nanjing, China.
⁵ Department of Otolaryngology Head and Neck Surgery, The Second Affiliated Hospital of Anhui Medical University, Hefei, China.
⁶ Materials Science and Devices Institute, Suzhou University of Science and Technology, Suzhou, China.
⁷ Department of Cardiovascular Surgery of the First Affiliated Hospital and Institute for Cardiovascular Science, Medical College, Soochow University, Suzhou, China.

Abstract

Neural stem cells (NSCs) transplantation is a promising approach for the treatment of various neurodegenerative diseases. Superparamagnetic iron oxide nanoparticles (SPIOs) are reported to modulate stem cell behaviors and are used for medical imaging. However, the detailed effects of SPIOs under the presence of static magnetic field (SMF) on NSCs are not well elucidated. In this study, it was found that SPIOs could enter the cells within 24 h, while they were mainly distributed in the lysosomes. SPIO exhibited good adhesion and excellent biocompatibility at concentrations below 500 μg/ml. In addition, SPIOs were able to promote NSC proliferation in the absence of SMF. In contrast, the high intensity of SMF (145 ± 10 mT) inhibited the expansion ability of NSCs. Our results demonstrate that SPIOs with SMF could promote NSC proliferation, which could have profound significance for tissue engineering and regenerative medicine for SPIO applications.

Keywords: SMF; SPIO; neural stem cells; proliferation; regulation.