CLIPSing Melanotan-II to Discover Multiple Functionally Selective hMCR Agonists

J Med Chem. 2022 Mar 10;65(5):4007-4017. doi: 10.1021/acs.jmedchem.1c01848. Epub 2022 Feb 21.

Abstract

The pleiotropic role played by melanocortin receptors (MCRs) in both physiological and pathological processes has stimulated medicinal chemists to develop synthetic agonists/antagonists with improved potency and selectivity. Here, by deploying the Chemical Linkage of Peptide onto Scaffolds strategy, we replaced the lactam cyclization of melanotan II (MT-II), a potent and unselective agonist of human MCRs (hMCRs), with different xylene-derived thioethers. The newly designed peptides displayed binding affinities toward MCRs ranging from the low nanomolar to the sub-micromolar range, highlighting a correlation between the explored linkers and the affinity toward hMCRs. In contrast to the parent peptide (MT-II), compound 5 displayed a remarkable functional selectivity toward the hMC1R. Enhanced sampling molecular dynamics simulations were found to be instrumental in outlining how the employed cyclization strategy affects the peptides' conformational behavior and, as a consequence, the detected hMC1R affinity. Additionally, a model of the peptide 5/hMC1R complex employing the very recently reported cryogenic electron microscopy receptor structure was provided.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Humans
  • Peptides, Cyclic / chemistry
  • Peptides, Cyclic / pharmacology
  • Receptors, Melanocortin* / chemistry
  • Structure-Activity Relationship
  • alpha-MSH* / analogs & derivatives
  • alpha-MSH* / chemistry

Substances

  • Peptides, Cyclic
  • Receptors, Melanocortin
  • melanotan-II
  • alpha-MSH