The combination of aerobic and microaerobic promote hydrolysis and acidification of rice straw and pig manure: Balance of insoluble and soluble substrate

Separated hydrolysis and acidification is an effective pretreatment method for anaerobic digestion of lignocellulose. However, excess consumption of soluble substrates remains a problem. Rice straw and pig manure were used as substrates with biogas slurry as the inoculum, combined with aerobic and microaerobic conditions in the 14-day hydrolysis and acidification. Aeration can significantly accelerate volatile solid degradation (38.25%), especially the lignocellulose. Soluble chemical oxygen demand (29157 mg/L) and volatile fatty acids (13219 mg/L) of the group with 4 days aerobic treatment, reached their peaks on day 5, obtaining a balanced insoluble substrate degradation and soluble substrate consumption. Candida, Lactobacillus, Bifidobacterium, and Acetobacter were enriched at the balanced point for positive contribution to the degradation of the insoluble substrate and the generation of soluble substrate. This study not only reveals the balance between degradation and consumption, but also provides new insight into biogas slurry recycling and anaerobic digestion precursor substrate production.