The m6A reader IGF2BP3 promotes acute myeloid leukemia progression by enhancing RCC2 stability

Exp Mol Med. 2022 Feb;54(2):194-205. doi: 10.1038/s12276-022-00735-x. Epub 2022 Feb 25.

Abstract

N6-methyladenosine (m6A) is the most abundant posttranscriptional modification of mRNA in eukaryotes. Recent evidence suggests that dysregulated m6A-associated proteins and m6A modifications play a pivotal role in the initiation and progression of diseases such as cancer. Here, we identified that IGF2BP3 is specifically overexpressed in acute myeloid leukemia (AML), a subtype of leukemia associated with poor prognosis and high genetic risk. IGF2BP3 is required for maintaining AML cell survival in an m6A-dependent manner, and knockdown of IGF2BP3 dramatically suppresses the apoptosis, reduces the proliferation, and impairs the leukemic capacity of AML cells in vitro and in vivo. Mechanistically, IGF2BP3 interacts with RCC2 mRNA and stabilizes the expression of m6A-modified RNA. Thus, we provided compelling evidence demonstrating that the m6A reader IGF2BP3 contributes to tumorigenesis and poor prognosis in AML and can serve as a target for the development of cancer therapeutics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine / metabolism
  • Apoptosis / genetics
  • Chromosomal Proteins, Non-Histone
  • Guanine Nucleotide Exchange Factors
  • Humans
  • Leukemia, Myeloid, Acute* / genetics
  • Leukemia, Myeloid, Acute* / metabolism
  • RNA
  • RNA, Messenger / genetics
  • RNA-Binding Proteins / metabolism*

Substances

  • Chromosomal Proteins, Non-Histone
  • Guanine Nucleotide Exchange Factors
  • RCC2 protein, human
  • RNA, Messenger
  • RNA-Binding Proteins
  • RNA
  • Adenosine