Elimination of Cas9-dependent off-targeting of adenine base editor by using TALE to separately guide deaminase to target sites

Yang Liu; Jizeng Zhou; Ting Lan; Xiaoqing Zhou; Yang Yang; Chuan Li; Quanjun Zhang; Min Chen; Shu Wei; Shuwen Zheng; Lingyin Cheng; Yuling Zheng; Liangxue Lai; Qingjian Zou

doi:10.1038/s41421-022-00384-4

Elimination of Cas9-dependent off-targeting of adenine base editor by using TALE to separately guide deaminase to target sites

Cell Discov. 2022 Mar 23;8(1):28. doi: 10.1038/s41421-022-00384-4.

Authors

Yang Liu^#^{1

2

3}, Jizeng Zhou^#⁴, Ting Lan^{1

5}, Xiaoqing Zhou², Yang Yang⁴, Chuan Li², Quanjun Zhang^{1

6

7}, Min Chen², Shu Wei⁸, Shuwen Zheng², Lingyin Cheng², Yuling Zheng², Liangxue Lai^{9

10

11

12}, Qingjian Zou¹³

Affiliations

¹ CAS Key Laboratory of Regenerative Biology, Guangdong-Hong Kong Stem Cell and Regenerative Medicine Research Centre, Joint Research Laboratory on Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China.
² Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, School of Biotechnology and Health Sciences, Wuyi University, Jiangmen, China.
³ School of Life Sciences, University of Science and Technology of China, Hefei, China.
⁴ School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou, China.
⁵ University of Chinese Academy of Sciences, Beijing, China.
⁶ Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory), Guangzhou, China.
⁷ Research Unit of Generation of Large Animal Disease Models, Chinese Academy of Medical Sciences (2019RU015), Guangzhou, China.
⁸ State Key Laboratory of Conservation and Utilization of Bio-Resources in Yunnan and Center for Life Science, School of Life Sciences, Yunnan University, Kunming, China.
⁹ CAS Key Laboratory of Regenerative Biology, Guangdong-Hong Kong Stem Cell and Regenerative Medicine Research Centre, Joint Research Laboratory on Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China. [email protected].
¹⁰ Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, School of Biotechnology and Health Sciences, Wuyi University, Jiangmen, China. [email protected].
¹¹ Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory), Guangzhou, China. [email protected].
¹² Research Unit of Generation of Large Animal Disease Models, Chinese Academy of Medical Sciences (2019RU015), Guangzhou, China. [email protected].
¹³ Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, School of Biotechnology and Health Sciences, Wuyi University, Jiangmen, China. [email protected].

^# Contributed equally.

No abstract available

Publication types

Letter