Pentachlorophenol has significant adverse effects on hematopoietic and immune system development in zebrafish (Danio rerio)

PLoS One. 2022 Mar 25;17(3):e0265618. doi: 10.1371/journal.pone.0265618. eCollection 2022.

Abstract

In November 2018, the Camp Fire devastated the mountain community of Paradise, CA. The burning of plastic pipes, wiring, construction materials, paint, and car batteries released toxic chemicals into the environment, contaminating the air, soil, and local waterways. Examples of toxins that were identified in the creeks and waterways in and around Paradise included pentachlorophenol (PCP), chrysene, and polyaromatic hydrocarbons. The effects of some of these chemicals on embryonic development, hematopoiesis (blood formation), and the immune system have not been thoroughly studied. Defining safe levels and the long-term effects of exposure is imperative to understanding and mitigating potential negative future outcomes. To perform these studies, we utilized zebrafish (Danio rerio), a commonly used vertebrate model system to study development. We observed the adverse effects of PCP on the development of zebrafish by using fluorescence microscopy, and saw that increased concentrations of PCP decreased the numbers of normal red blood cells and myeloid cells. Additionally, we observed that animal survival decreased in response to increasing concentrations of PCP. Furthermore, the prevalence of characteristic physical deformities such as tail curvature were greater in the treatment groups. Lastly, runx1, cmyb, and cd41 expression was reduced in fish treated with PCP. These results suggest that PCP has a previously underappreciated effect on blood and immune cell development and future studies should be performed to determine the molecular mechanisms involved.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Embryonic Development
  • Hematopoiesis
  • Organogenesis
  • Pentachlorophenol* / toxicity
  • Zebrafish

Substances

  • Pentachlorophenol

Grants and funding

This research was supported by a grant from the CSU Council on Ocean Affairs, Science & Technology (COAST) to W.D and A.N. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.