Quantification of Plasmodesmata Permeability in Arabidopsis Leaves by Tracing the Movement of GFP

Min Diao; Shanjin Huang

doi:10.1007/978-1-0716-2132-5_21

Quantification of Plasmodesmata Permeability in Arabidopsis Leaves by Tracing the Movement of GFP

Methods Mol Biol. 2022:2457:313-320. doi: 10.1007/978-1-0716-2132-5_21.

Authors

Min Diao¹, Shanjin Huang²

Affiliations

¹ iHuman Institute, Shanghai Tech University, Shanghai, China.
² Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing, China. [email protected].

PMID: 35349150
DOI: 10.1007/978-1-0716-2132-5_21

Abstract

Plasmodesmata (PD) play an important role in plant growth and development and defense. The permeability of PD is strictly regulated. Here, we describe an assay for measuring the permeability of PD in Arabidopsis thaliana leaves, which relies on tracing intercellular movement of green fluorescent protein (GFP) upon transient expression of the protein-encoding plasmid delivered by particle bombardment. The method allows to assess GFP movement at single-cell resolution.

Keywords: GFP; PD permeability; Particle bombardment; Plasmodesmata.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Arabidopsis* / genetics
Arabidopsis* / metabolism
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
Nicotiana / metabolism
Permeability
Plant Leaves / genetics
Plant Leaves / metabolism
Plasmodesmata* / metabolism

Substances

Green Fluorescent Proteins