Abstract
Transin RNA is a 1.9-kilobase RNA transcript induced by oncogenes in rat embryo fibroblast cell lines. We show that RNA species complementary to a cloned transin cDNA are present in mouse skin squamous cell carcinomas induced by a classical initiation-promotion protocol but not in premalignant, benign papillomas or in normal epidermis. A single application of a tumor promoting phorbol ester to normal epidermis elicits a transient increase in these RNA levels. Transin RNA encodes a secreted protease, an activity consistent with a functional role for enhanced expression of transin RNA in the progression of benign, encapsulated tumors to malignant, invasive carcinomas.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Carcinoma, Squamous Cell / enzymology
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Carcinoma, Squamous Cell / genetics
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Carcinoma, Squamous Cell / pathology
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Cell Transformation, Viral
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Endopeptidases / genetics*
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Gene Expression Regulation
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Matrix Metalloproteinase 3
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Mice
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Neoplasm Proteins / genetics*
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Neoplasms, Experimental / enzymology
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Neoplasms, Experimental / genetics*
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Neoplasms, Experimental / pathology
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Papilloma / enzymology
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Papilloma / genetics
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Papilloma / pathology
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RNA, Messenger / genetics
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RNA, Neoplasm / genetics*
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Skin Neoplasms / enzymology
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Skin Neoplasms / genetics
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Skin Neoplasms / pathology
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Tetradecanoylphorbol Acetate / pharmacology
Substances
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Neoplasm Proteins
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RNA, Messenger
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RNA, Neoplasm
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Endopeptidases
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Matrix Metalloproteinase 3
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Tetradecanoylphorbol Acetate