Identification of new Omega-3 very long chain poly-unsaturated fatty acids in meibomian gland secretions

Romain Magny; Anne Regazzetti; Karima Kessal; Orane Christin; Christophe Baudouin; Emmanuel Roulland; Françoise Brignole-Baudouin; Olivier Laprévote; Nicolas Auzeil

doi:10.1016/j.biochi.2022.04.008

Identification of new Omega-3 very long chain poly-unsaturated fatty acids in meibomian gland secretions

Biochimie. 2022 Dec:203:3-10. doi: 10.1016/j.biochi.2022.04.008. Epub 2022 Apr 25.

Authors

Romain Magny¹, Anne Regazzetti², Karima Kessal³, Orane Christin², Christophe Baudouin⁴, Emmanuel Roulland², Françoise Brignole-Baudouin⁵, Olivier Laprévote⁶, Nicolas Auzeil⁷

Affiliations

¹ Sorbonne Université UM80, INSERM UMR 968, CNRS UMR 7210, Institut de la Vision, IHU ForeSight, 75012, Paris, France; Université Paris Cité, CNRS, CiTCoM, F-75006, Paris, France. Electronic address: [email protected].
² Université Paris Cité, CNRS, CiTCoM, F-75006, Paris, France.
³ Sorbonne Université UM80, INSERM UMR 968, CNRS UMR 7210, Institut de la Vision, IHU ForeSight, 75012, Paris, France; CIC 1423, Centre Hospitalier National d'Ophtalmologie des Quinze-Vingts, IHU ForeSight, 75012, Paris, France.
⁴ Sorbonne Université UM80, INSERM UMR 968, CNRS UMR 7210, Institut de la Vision, IHU ForeSight, 75012, Paris, France; CIC 1423, Centre Hospitalier National d'Ophtalmologie des Quinze-Vingts, IHU ForeSight, 75012, Paris, France; Hôpital Ambroise Paré, AP-HP, Université Versailles St Quentin en Yvelines, Paris Saclay, 78180, Montigny-Le-Bretonneux, France.
⁵ Sorbonne Université UM80, INSERM UMR 968, CNRS UMR 7210, Institut de la Vision, IHU ForeSight, 75012, Paris, France; Université Paris Cité, CNRS, CiTCoM, F-75006, Paris, France; Laboratoire d'ophtalmobiologie, Centre Hospitalier National d'Ophtalmologie des Quinze-Vingts, IHU ForeSight, 75012, Paris, France.
⁶ Université Paris Cité, CNRS, CiTCoM, F-75006, Paris, France; Hôpital Européen Georges Pompidou, AP-HP, Service de Biochimie, 75015, Paris, France.
⁷ Université Paris Cité, CNRS, CiTCoM, F-75006, Paris, France. Electronic address: [email protected].

PMID: 35476941
DOI: 10.1016/j.biochi.2022.04.008

Abstract

Three new very long chain polyunsaturated fatty acids (VLC PUFA) belonging to the omega-3 family have been identified in meibum samples collected by Schirmer strips. These VLC PUFA, namely FA (32:3), FA (34:3) and FA (36:3), were detected in O-acyl-ω-hydroxy fatty acids using a molecular network approach, and as free fatty acids. Identification was supported by retention time prediction model, exact mass determination and isotopic patterns. Double bond location was determined using cross metathesis reaction associated to tandem mass spectrometry. In meibum, synthesis of these VLC PUFA is likely to be mediated by elongation of very long chain fatty acids 4 enzyme. The biological role of these newly VLC PUFA and their occurrence in other tissues and biological fluids remains to be elucidated.

Keywords: Cross metathesis; Lipid identification; Meibum; Molecular network; Ocular surface; Tandem mass spectrometry.

MeSH terms

Fatty Acids / chemistry
Fatty Acids, Omega-3*
Meibomian Glands* / chemistry
Tandem Mass Spectrometry

Substances

Fatty Acids
Fatty Acids, Omega-3