The hematopoietic compartment is sufficient for lupus development resulting from the POLB-Y265C mutation

PLoS One. 2022 Apr 29;17(4):e0267913. doi: 10.1371/journal.pone.0267913. eCollection 2022.

Abstract

Systemic lupus erythematosus is a chronic disease characterized by autoantibodies, renal and cutaneous disease, and immune complex formation. Emerging evidence suggests that aberrant DNA repair is an underlying mechanism of lupus development. We previously showed that the POLBY265C/C mutation, which results in development of an aberrant immune repertoire, leads to lupus-like disease in mice. To address whether the hematopoietic compartment is sufficient for lupus development, we transplanted bone marrow cells from POLBY265C/C and POLB+/+ into wild-type congenic mice. Only mice transplanted with the POLBY265C/C bone marrow develop high levels of antinuclear antibodies and renal disease. In conclusion, we show that the hematopoietic compartment harvested from the POLBY265C/C mice is sufficient for development of autoimmune disease.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Antibodies, Antinuclear / genetics
  • Autoantibodies / genetics
  • DNA Polymerase beta / metabolism*
  • Lupus Erythematosus, Systemic* / genetics
  • Mice
  • Mutation

Substances

  • Antibodies, Antinuclear
  • Autoantibodies
  • DNA Polymerase beta
  • DNA polymerase beta, mouse