Investigating Plant Biosynthetic Pathways Using Heterologous Gene Expression: Yeast as a Heterologous Host

Methods Mol Biol. 2022:2489:369-393. doi: 10.1007/978-1-0716-2273-5_19.

Abstract

Plant natural products (PNPs) are valuable resources for the development of pharmaceuticals and agrochemicals, yet the biosynthesis and metabolism of PNPs are largely unknown. Heterologous pathway reconstitution is a heavily adopted strategy in secondary metabolism characterization. Yeast systems have been broadly utilized in the heterologous production of PNPs and have been considered as a promising platform to investigate plant biosynthetic pathways. Here, we describe the reconstitution and verification of the upstream part of brassinolide biosynthesis in S. cerevisiae using this method.

Keywords: Biosynthesis; Heterologous gene expression; Metabolic engineering; Natural products; Saccharomyces cerevisiae; Yeast.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biological Products* / metabolism
  • Biosynthetic Pathways* / genetics
  • Gene Expression
  • Metabolic Engineering / methods
  • Plants / genetics
  • Plants / metabolism
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism

Substances

  • Biological Products